Gas Chromatography interview questions and answers | GC interview questions | GC FAQ | English Excel
Gas chromatography interview questions and answers are somewhere that kept us on the backfoot while interviewing. In this article, we will try to cover such Gas chromatography interview questions.
Answer: This question could be tricky but let’s understand it in a simple way, gas chromatography works on different factors such as temperature, gas flow, and column length. Because of factors affecting retention time, it is important that, If the gas chromatography is having a specified method then it will be easy to identify the peaks in gas chromatography and understand the elution pattern of peaks.
Answer: When the sample solution is injected through the injection port, the sample is entered into a column. Due to the high temperature in the column, the sample gets evaporated and travels through the column with the mobile phase. The temperature (Temperature Program) is set in such a way that the component should get a response at its Boiling point only. The components which are having a lower boiling point will elute first as compared to high boiling point components.
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In this parameter of method validation, the range of 0-150% of the expected level of analysis should be covered. The method should display linearity in the desired range. For the linearity purpose, prepare six samples in desire range to take linearity and correlation coefficient value should be minimum R² = 0.99.
In chromatography analysis, if the sample having more than one compounds then need to be separate properly. If the retention time of two peaks have adequately different or peaks are sharp then get better resolution. The resolution shows the separation and sharpness of peak, an acceptable limit of resolution are Rs >1.5.
Specificity is a method validation parameter, Spike your sample in addition with degradation products, solvents, chemicals and solvents used to perform analysis, hence all analytes should be separate and minimum resolution have 2.0. and another one is to spike samples of marketed preparation, it should be separate having RSD ≤ 2 %.
Method validation in chromatography must be carried out as per the validation protocol is given by the International Council for Harmonization of Technical Requirements for Pharmaceuticals for Human Use (ICH) or The United States Pharmacopeia (USP) and British Pharmacopoeia (BP). The protocol includes the process and criteria of acceptance for all protocol.
Column chromatography is a general technique that is used to separate the compounds from the complex mixtures. It can use small or large-scale column chromatography to separate and purify the analytes. Column chromatography has two phases, a mobile phase (liquid) and a stationary phase (solid). The sample mixture of compounds travels with the mobile phase through the stationary phase and basis of different degrees of adhesion it separates.The column chromatography works on the principle of adsorption. There are four different column chromatography types for several applications and that work on different mechanisms.
Best GAS CHROMATOGRAPHY Interview Questions with Answers
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FAQ
What are the components of GCMS?
What is the difference between GC and GCMS?
What are the GC calibration parameters?
- Retention Time. Retention time refers to the time a compound is retained in the gas chromatography column. …
- Size of the peaks of the spectra. Peak size is another parameter that is used to present the results of gas chromatography. …
- Mass-to-charge Ratio (m/z)
